In his first public address, Mahn Win Khaing Than said "this is the darkest moment of the nation and the moment that the dawn is close".
In his speech on Facebook, Mahn Win Khaing Than said: "This is the time for our citizens to test their resistance against the dark moments.
"In order to form a federal democracy, which all ethnic brothers, who have been suffering various kinds of oppressions from the dictatorship for decades... this revolution is the chance we can put our efforts together."
From hiding, Mahn Win Khaing Than leads a group of legislators who have refused to accept last month's coup.
As many as 12 protesters are reported to have been killed on Saturday.
Myanmar (also known as Burma) has been gripped by street protests since the military seized control on 1 February and detained Aung San Suu Kyi, the leader of the National League for Democracy (NLD) party.
The NLD won a landslide victory in last year's election, but the military said the poll was fraudulent.
NLD MPs who managed to escape arrest formed a new group, the Committee for Representing Pyidaungsu Hluttaw (CRPH), with Mahn Win Khaing Than appointed acting head. The CRPH is seeking international recognition as Myanmar's rightful government.